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In the arcuate center, GHS-R mRNA was revealed in 94 ± 1% of the nerve cells expressing NPY, 8 ± 2% of those expressing POMC and 30 ± 6% sharing SRIH mRNA. 20-- 25% of the GHRH- mRNA-expressing neurons contained GHS-R mRNA, whereas the vast bulk of the arcuate GHS-R-mRNA-containing cells did not include GHRH mRNA. The finding of a significant co-expression of GHS-R and NPY mRNA in the arcuate core is in conformity with the previous demo by Dickson et al. that c-Fos is caused in NPY neurons following GHS administration. These results show that GHS have other impacts on neuroendocrine regulation than GH launch using GHRH nerve cells.<br>However, in spite of decreases in signaling when made use of in cell-based assays, these GHSR1a villains had unanticipated impacts in pet designs. In both rats and dogs GHSR1a antagonists decrease GH secretion but stimulate hunger (Costantini et al., 2011; Hassouna et al., 2013). 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